Micro Test for Streptococcal Anti-deoxyribonuclease B

Micro test for streptococcal anti-deoxyribonuclease B.

A methyl green decolorization micro test for the determination of streptococcal anti-deoxyribonuclease B titers in serum is described. The micro test was compared with an alcohol precipitation macro test on 112 human sera. The anti-deoxyribonuclease B titers agreed within one dilution step for 85.7% of the 112 sera examined by both techniques. Both tests were about equal in the detection of tit...

Extremely high titers of serum antibodies against the streptococcal exoenzyme deoxyribonuclease B.

In sera from 4 of 25,000 individuals tested for antibodies against streptococci, extremely high antideoxyribonuclease B titers ( > 10(6) U/ml) were found. Two of the cases were diagnosed as monoclonal gammopathies. The M-components were shown to possess the anti-deoxyribonuclease B activity. The other two cases were diagnosed as relapsing erysipelas. The high serum titers of deoxyribonuclease B...

Evaluation of the Wampole Streptonase B test.

The Streptonase B test was compared with the Center for Disease Control streptococcal anti-deoxyribonuclease B test. A total of 96% of anti-deoxyribonuclease B titers obtained with the Streptonase B test were within +/- 1 dilution step of those obtained with the Center for Disease Control anti-deoxyribonuclease B test.

Differentiation of ribonucleic acids by inhibition of streptococcal deoxyribonuclease.

It has been shown (Bernheimer & Ruffier, 1951 ) that physical disruption of streptococci belonging to Lancefield groups A, B, C and D results in the liberation of a substance which partially inhibits the depolymerizing action of group A streptococcal deoxyribonuclease. One of the striking characteristics of the inhibitory substance is its specificity, for although it inhibits the deoxyribonucle...

Saline test for streptococcal haemolysin.